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目的探讨大鼠胚胎成纤维细胞移植对深Ⅱ度烧伤创面愈合的影响。方法取成年Wistar大鼠48只,在每只大鼠背部制作做2个深Ⅱ度烧伤创面,磨痂去除坏死组织,按自身对照原则,将创面分为细胞移植组(A组,n=48),对照组(B组,n=48)。将羰花青荧光染料CM-dil标记的胚胎成纤维细胞移植到A组创面组织内,B组注射同量的成体成纤维细胞。分别于伤后0、1、3、7、14、28 d不同时间各取8只大鼠进行实验,观察荧光信号的分布范围比例A(%)及信号强度比D的变化,记录两组创面愈合的时间T,观察两组创面组织TGF-β1 mRNA表达的变化。结果伤后3 d荧光分布范围开始扩展(A:15.46±1.21),荧光强度开始降低(D:0.65±0.02);第14天荧光分布范围扩展到最大(A:16.69±1.00),荧光强度的信号更加降低(D=0.31±0.01)。第28天亦能检测到微弱的荧光信号(D=0.09±0.01),分布范围较伤后14 d无明显扩展(16.07±1.14)%。A组TGF-β1 mRNA水平伤后即升高,1 d后开始下降。B组TGF-β1 mRNA伤后持续升高,第7天达到峰值,其后回落,在观察时间其表达量一直高于A组。结论大鼠胚胎成纤维细胞移植可以促进深Ⅱ度烧伤创面愈合,降低TGF-β1 mRNA的表达,改善创面愈合,减轻瘢痕的形成。
Objective To investigate the effect of rat embryo fibroblast transplantation on deep second degree burn wound healing. Methods Forty-eight adult Wistar rats were made on the back of each rat. Two deep second degree burn wounds were made on the back of each rat. The necrotic tissue was removed by grinding scab. According to the self-control principle, the wounds were divided into cell transplantation group (group A, n = 48 ), Control group (group B, n = 48). Embryonic fibroblasts labeled with carbocyanine fluorescent dye CM-dil were transplanted into wound tissue of group A, and group B injected with the same amount of adult fibroblasts. The rats were sacrificed on days 0, 1, 3, 7, 14 and 28 after injury respectively. Eight rats were sacrificed at different time points to observe the changes of fluorescence signal distribution ratio A (%) and signal intensity ratio D, The healing time T, observe the changes of TGF-β1 mRNA expression in the two wounds. Results After 3 days, the fluorescence distribution began to expand (A: 15.46 ± 1.21), and the fluorescence intensity began to decrease (D: 0.65 ± 0.02). On the 14th day, the fluorescence distribution range expanded to the maximum (A: 16.69 ± 1.00) The signal was more reduced (D = 0.31 ± 0.01). Weak fluorescent signal (D = 0.09 ± 0.01) was also detected on the 28th day, with no significant expansion (16.07 ± 1.14)% on the 14th day. A group of TGF-β1 mRNA levels increased immediately after injury, began to decline after 1 d. B group TGF-β1 mRNA sustained increased after injury, peaked on the 7th day, and then down, at the time of observation its expression was consistently higher than the A group. Conclusion Rat embryo fibroblast transplantation can promote wound healing in deep second degree burn wounds, decrease the expression of TGF-β1 mRNA, improve wound healing and reduce the formation of scar.