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目的观察人重组白细胞介素24(rhIL-24)和表没食子儿茶素没食子酸酯(EGCG)对组织新生血管和肿瘤组织血管生成的影响并作比较。方法在鸡胚模型上比较观察rhIL-24、EGCG对鸡胚绒毛尿囊膜(CAM)血管生成的影响,在人肺腺癌裸鼠移植瘤模型上比较观察两者对肿瘤组织生长、组织中CD34和NF-κB(p50)的表达影响。结果rhIL-24、EGCG均可抑制CAM上Ⅱ、Ⅲ级血管的生成。rhIL-24组瘤体组织中NF-κB(p50)的表达与PBS组比较有显著性差异,而EGCG组则不明显;rhIL-24组和EGCG组瘤体组织中CD34标记的微血管密度,与PBS组比较均有显著性差异。结论rhIL-24、EGCG都有抑制CAM血管生成和肿瘤组织血管生成的作用,对肿瘤组织中NF-κB表达可能存在不同影响,推测两者或许存在不同的抗肿瘤血管生成机制。
Objective To observe the effects of recombinant human interleukin 24 (rhIL-24) and epigallocatechin-3-gallate (EGCG) on angiogenesis in new blood vessels and tumor tissues. Methods The effect of rhIL-24 and EGCG on angiogenesis of chick chorioallantoic membrane was observed in chicken embryo model. The growth of tumor tissue was observed in the model of human lung adenocarcinoma xenograft in nude mice. CD34 and NF-κB (p50) expression. Results Both rhIL-24 and EGCG inhibited the formation of grade II and grade III vessels in CAM. The expression of NF-κB (p50) in rhIL-24 group was significantly different from that in PBS group, but not in EGCG group. The density of CD34-labeled microvessel in the tumor tissue of rhIL-24 group and EGCG group was PBS group compared with significant differences. Conclusion Both rhIL-24 and EGCG can inhibit angiogenesis of CAM and angiogenesis of tumor tissue, and may have different effects on the expression of NF-κB in tumor tissue. It is speculated that there may exist different anti-tumor angiogenesis mechanisms between them.