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目的探讨羌活提取物(EN)对哮喘小鼠Thl/Th2细胞平衡的影响,以及p38信号通路在其中的作用。方法 60只雄性清洁级BABL/c小鼠,随机分为6组,每组10只,分别为正常对照组;卵蛋白(OVA)哮喘组;羌活低剂量组;羌活高剂量组、SB203580组和地塞米松组。在末次激发24 h后小鼠肺组织行HE染色。分别用ELISA检测支气管肺泡灌洗液(BALF)中白细胞介素(IL)-4、IL-5、IL-13和γ-干扰素(IFN-γ)含量以及Western blotting检测肺组织IL-4、IFN-γ、P38蛋白表达。结果哮喘组小鼠与对照组小鼠相比较,BALF中炎症细胞计数、IL-4、IL-5、IL-13水平增高,而IFN-γ的表达明显降低(P<0.05),肺部炎症浸润明显(P<0.05);肺组织IL-4和磷酸化P38蛋白表达水平显著高于对照组(P<0.05),而肺组织IFN-γ的表达明显低于对照组(P<0.05)。羌活低、高剂量组、SB203580组和地塞米松组与哮喘模型组相比较,BALF中炎症细胞计数、IL-4、IL-5、IL-13浓度以及肺组织IL-4和磷酸化P38蛋白表达水平均显著降低(P<0.05);肺部炎症明显减轻(P<0.05);BALF和肺组织中IFN-γ明显升高(P<0.05)。结论羌活提取物具有明显的抗哮喘作用,其机制可能是通过抑制P38信号通路,影响Thl/Th2细胞平衡实现的。
Objective To investigate the effect of Notopterygium extract on the balance of Th1 / Th2 cells in asthmatic mice and its role in p38 signaling pathway. Methods Sixty male BABL / c male mice were randomly divided into 6 groups (n = 10): normal control group, ovalbumin (OVA) asthma group, low activity of Notopterygium, high activity of Notopterygium, Dexamethasone group. Mouse lung tissue was stained with HE 24 h after the last challenge. The levels of IL-4, IL-5, IL-13 and IFN-γ in bronchoalveolar lavage fluid (BALF) were detected by ELISA and the levels of IL-4, IFN-γ, P38 protein expression. Results The number of inflammatory cells, the levels of IL-4, IL-5 and IL-13 in BALF and the expression of IFN-γ in BALF were significantly lower in asthmatic mice than in control mice (P <0.05) (P <0.05). The expression of IL-4 and phosphorylated P38 protein in lung tissue was significantly higher than that in control group (P <0.05), while the expression of IFN-γ in lung tissue was significantly lower than that in control group (P <0.05). Compared with the asthma model group, the inflammatory cell counts, the concentrations of IL-4, IL-5 and IL-13 in BALF and the levels of IL-4 and phosphorylated P38 protein in lung tissue of SB203580 group and dexamethasone group (P <0.05). The inflammation of the lungs was significantly reduced (P <0.05). The levels of IFN-γ in BALF and lung tissue were significantly increased (P <0.05). Conclusion Rhizoma et Radix notoginseng extract has a significant anti-asthmatic effect. The mechanism may be through inhibiting the P38 signaling pathway and affecting the balance of Thl / Th2 cells.