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目的对重庆市人群沙门菌感染进行监测并对监测分离的菌株进行耐药性和脉冲场凝胶电泳(PFGE)分子分型研究。方法明确监测病例定义,制定、实施监测方案,实验室分离、鉴定监测的沙门菌,利用K-B法对分离菌株进行药敏检测,并采用PFGE进行分子分型。结果2007年监测了835例病例,病例分布在9个区(县),其中监测沙门菌暴发疫情10起,病例175例。实验室共分离鉴定了79株12种沙门菌(11种血清型和1组B群),全部菌株对至少一种抗菌素耐药,9种(75%)对3种以上的抗菌素耐药,2种(16.67%)对6种抗菌素耐药,尤其是里定沙门菌、婴儿沙门菌及汤卜逊沙门菌耐药严重。沙门菌PFGE分型被分成9种14种亚型。结论重庆市2007年人群沙门菌分离株没有明显的优势流行株,同一事件中的沙门菌PFGE分型相同,分离株耐药情况严重,分离菌株的耐药谱和PFGE型也没有明显的联系。
Objective To monitor the population of Salmonella in Chongqing and analyze the drug resistance and PFGE molecular typing of the isolates. Methods Clearly define the case definition, develop and implement monitoring programs, and separate and identify the Salmonella strains in laboratories. K-B method was used to detect the susceptibility of the isolated strains and PFGE was used for molecular typing. Results A total of 835 cases were monitored in 2007 and the cases were distributed in 9 districts (counties). Outbreaks of Salmonella were detected in 10 cases and 175 cases. A total of 79 strains of 12 Salmonella species (11 serotypes and 1 group B) were isolated and identified in the laboratory. All strains were resistant to at least one antibiotic, 9 (75%) were resistant to 3 or more antibiotics, 2 Six species (16.67%) were resistant to six antibiotics, especially Salmonella typhimurium, Salmonella babies and Salmonella tybus. Salmonella PFGE typing is divided into nine subtypes of 14 subtypes. Conclusion There was no obvious prevalent epidemic strain of Salmonella isolates in Chongqing in 2007, and the same type of Salmonella PFGE was found in the same incident. The drug resistance of the isolated strains was serious. There was no obvious relationship between the resistant strains and PFGE type of isolates.