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在三年云南楚雄克山病综合考察中,几个单位通过不同手段对收集到的克山病死亡标本,对心肌的呼吸酶活性等项目进行了测试研究。本所用 Mason 方法对心肌细胞色素氧化酶(CCO活性,通过 Unicam SP-1800紫外分光光度计联用循环水浴恒温吸收池测定还原细胞色素 C 的氧化速度的方法,对酶活性及其一级反应速度常数进行了测定。结果表明,亚急型克山病(共8例)的 CCO 活性最低,慢型(5例)亦低于非心肌病死亡者心肌的酶活性。与此同时,作为线粒体内膜结合酶必要的结合脂质(界面脂)—心磷脂(Cardiolipin)减少。
In the three years of Yunnan Chuxu Keshan disease comprehensive inspection, several units through different means to collect the Keshan disease death specimens, the myocardial respiratory enzyme activity and other projects were tested. The use of Mason’s method of myocardial cytochrome oxidase (CCO activity, Unicam SP-1800 UV spectrophotometer with a circulating water bath thermostatic cell determination of the reduction of cytochrome C oxidation rate of enzyme activity and its first-order reaction rate The results showed that subacute Keshan disease (a total of 8 cases) had the lowest CCO activity, slow type (5 cases) was also lower than non-cardiomyopathy death myocardial enzyme activity.At the same time, as a mitochondrial The membrane-bound enzyme necessary for the reduction of the bound lipid (interfacial fat) -cardiolipin.