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目的：研究原发性肝细胞癌（ＨＣＣ）５ 对染色体３８ 个位点等位基因杂合性丢失（ＬＯＨ） ，ＬＯＨ 与肝癌的临床病理改变和肝炎病毒感染的关系。方法：用ＰＣＲ 微卫星多态性分析检测肝癌ＬＯＨ。结果：发生高频率ＬＯＨ 的位点有染色体１ｐ 的Ｄ１Ｓ１８６（ｐ３１ ， ４８－１％ ） 和Ｄ１Ｓ２４３（１ｐ３６－３ ， ５１－６ ％ ） ； 染色体９ｐ２４ 的Ｄ９Ｓ５４（６１－８％ ） 、９ｐ２１ 的Ｄ９Ｓ１７４７（５２－４％ ） 和ＤＤ９Ｓ１７５２（５１－８ ％ ） 位点；１０ 号染色体１０ｑ２３ ～２４ 区Ｄ１０Ｓ１２２３（５３－３ ％ ） 和Ｄ１０Ｓ１２２５（３８－７ ％ ）位点； １６ 号染色体１６ｑ２４ 区的Ｄ１６Ｓ４１３ 位点ＬＯＨ 频率最高（７０－８ ％ ） ； １７ 号染色体１７ｐ１３ 上ＴＰ５３（５３－８ ％ ） 和Ｄ１７Ｓ５２０（５６－８％ ） 。各染色体ＬＯＨ 与肝癌的临床病理改变无明显关系；ＨＣＶ 和ＨＢＶ 复合感染与ｐ５３ 和ｐ１５ＩＮＫ４Ｂ基因的ＬＯＨ 有关。结论： 肝癌的发生、发展与多染色体的ＬＯＨ 有关， 在染色体１ｐ３１ ， １ｐ３６～ｐ３５ ， ９ｐ２４ 和９ｐ２１ ，１０ｑ２２ ～２４ ，１６ｑ２４ 和１７ｐ 可能存在多个与ＨＣＣ 有关的ＴＳＧ。肝炎
Objective: To investigate the loss of heterozygosity (LOH) in 38 alleles of primary hepatocellular carcinoma (HCC) on chromosome 5, and the relationship between LOH and clinicopathological changes and hepatitis virus infection in hepatocellular carcinoma. Methods: Detection of LOH in hepatocellular carcinoma by PCR microsatellite polymorphism analysis. RESULTS: The loci with high frequency of occurrence of LOH were D1S186 (p31, 48-1%) and D1S243 (1p36-3, 51-6%) of chromosome 1p, D9S54 (61-8%) of chromosome 9p24, and D9S1747 of 9p21 ( 52-4%) and DD9S1752 (51-8 %) loci; chromosome 10 10q23 to 24 regions D10S1223 (53-3%) and D10S1225 (38-7 %) loci; chromosome 16 16q24 locus D16S413 The frequency of LOH was highest (70-8 %); 17p13 on chromosome 17 was TP53 (53-8 %) and D17S520 (56-8%). There was no significant relationship between the LOH of each chromosome and the clinicopathological changes of liver cancer; the combined infection of HCV and HBV was related to the LOH of p53 and p15INK4B genes. Conclusions: The occurrence and development of HCC are associated with multiple chromosomes of LOH. There may be multiple TSGs associated with HCC on chromosome 1p31, 1p36-p35, 9p24 and 9p21, 10q22-24, 16q24 and 17p. hepatitis