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为了提高普通的单基因ＰＣＲ检测致病性肠道杆菌的时效，采用ＥＴＥＣ４４８１３（ＬＴ）、ＥＴＥＣ１９４４９（ＳＴ）和福氏２ａＴ３２（ｉｐａＨ）３个标准株为模板，建立了检测产毒性大肠杆菌（ＬＴ，ＳＴ）和志贺氏菌的三基因ＰＣＲ方法。并分别用相应单基因ＰＣＲ方法标记的探针斑点杂交标准株ＥＴＥＣ４４８１５（ＬＴ）、工程株ＰＳＬＭ００４（ＳＴ）和标准株４８０２５１１（ｉｐａＨ）。杂交的结果都是阳性，证实了这个多基因ＰＣＲ扩增是特异的。并用此三基因ＰＣＲ反应系统检测１１３株ＥＴＥＣ和志贺氏菌，结果是ＬＴ２５株、ＳＴ１６株、ＬＴ和ＳＴ共３０株及ｉｐａＨ４２株。在几种病原体可能共存的样本中，用一次ＰＣＲ就能解决病原体的检测和鉴别问题，比单基因ＰＣＲ更快速、经济。
In order to improve the timeliness of detection of pathogenic enterobacter by single-gene PCR, we established the standard method for the detection of toxigenic E.coli (LT) by using three standard strains of ETEC44813 (LT), ETEC19449 (ST) and Freund’s 2aT32 (ipaH) , ST) and Shigella’s three-gene PCR method. The standard strain ETEC44815 (LT), engineering strain PSLM004 (ST) and standard strain 48025-11 (ipaH) were respectively labeled with the corresponding single-gene PCR method. The result of the hybridization is positive, confirming that this polygene PCR amplification is specific. 113 strains of ETEC and Shigella were detected by this three-gene PCR reaction system. The result was LT25 strain, ST16 strain, 30 LT and ST strains, and ipaH42 strain. In a sample where several pathogens may coexist, one-time PCR can solve the problem of pathogen detection and identification, faster and more economical than single-gene PCR.