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目的建立秦七风湿方的HPLC指纹图谱,为其质量评价及药效物质基础研究提供依据。方法采用Inert Sustain C18色谱柱(150 mm×4.6 mm,5μm);以0.1%磷酸水溶液-乙腈为流动相进行梯度洗脱;检测波长为203 nm;柱温为30℃;体积流量为1 m L/min;测定10批秦七风湿方样品,利用中药色谱相似度评价系统(2004A版)建立秦七风湿方HPLC指纹图谱,并通过对照品对共有峰进行指认。结果建立了秦七风湿方HPLC指纹图谱,10批样品的相似度均在0.99以上,共标定19个共有峰,8个峰来源于珠子参,8个峰来源于秦艽,5个峰来源于山茱萸(其中1号峰为珠子参、秦艽和山茱萸共有),并通过与对照品比较指认了其中8个色谱峰,分别为马钱苷酸(2号峰)、莫诺苷(3号峰)、龙胆苦苷(5号峰)、马钱苷(6号峰)、人参皂苷Ro(12号峰)、人参皂苷F1(13号峰)、竹节参皂苷IVa(14号峰)、人参皂苷F2(17号峰)。结论所建立的方法稳定、准确、重复性好,可为秦七风湿方的质量控制及药效物质基础研究提供依据。
Objective To establish the HPLC fingerprinting of QFD for providing the basis for its quality evaluation and pharmacodynamic basis. Methods Inert Sustain C18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase was eluted with 0.1% phosphoric acid and acetonitrile. The detection wavelength was 203 nm. The column temperature was 30 ℃. The volume flow rate was 1 m L / min; Determination of 10 batches of Qin and Qifen rheumatoid samples, the use of Chinese medicine chromatographic similarity evaluation system (2004A version) to establish Qin seven rheumatoid prescription HPLC fingerprinting, and through the reference to identify the common peak. Results The HPLC fingerprints of Qinshen Fengshuang were established. The similarity of the ten batches of samples were all above 0.99. The common peaks of 19 samples were obtained. The eight peaks were derived from Begonia ginseng, the eight peaks were from Gentiana macrophylla, the other five were from Cornus officinalis (No. 1 peak is common beads, Gentiana macrophylla and Cornus officinalis), and compared with the reference standard, 8 of them were identified, including racemarin (No.2), Novozone (No.3) Gentiopicroside (peak 5), loganin (peak 6), ginsenoside Ro (peak 12), ginsenoside F1 (peak 13), bamboo ginsenoside IVa (peak 14), ginsenoside F2 (peak 17). Conclusion The established method is stable, accurate and reproducible. It can provide the basis for the quality control and pharmacodynamic basis research of QFD.