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目的:研究乙醇提取蜂胶中总黄酮的最佳工艺。方法:以乙醇浓度、料液比、提取时间以及提取次数为考察因素,用L9(34)正交表设计安排正交试验,以总黄酮含量,白杨素、高良姜素的含量为考察指标,采用综合加权评分法进行数据分析,优选最佳提取工艺。结果:最佳工艺参数为:加入蜂胶量10倍90%乙醇,浸提三次,每次24 h。结论:该工艺简单、可行,可用于大规模生产。
Objective: To study the best technology of ethanol extraction of total flavonoids in propolis. Methods: Taking ethanol concentration, solid-to-liquid ratio, extraction time and extraction times as the investigation factors, the orthogonal experiment was designed by L9 (34) orthogonal design. The contents of total flavonoids, Data were analyzed by comprehensive weighted score method, and the best extraction process was optimized. Results: The optimum parameters were as follows: adding propolis 10 times 90% ethanol and leaching three times for 24 h. Conclusion: The process is simple and feasible and can be used for mass production.