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目的：应用秀丽隐杆线虫评估重金属生物毒性效应。方法将L4期秀丽隐杆线虫暴露于低浓度的CdCl2、CrCl3、As2O3、PbCl2和HgCl2溶液及M9缓冲液（对照组）中72 h，评价不同浓度重金属暴露对线虫生存时间和生殖毒性的影响。结果2.5、10μmol/L的HgCl2和PbCl2、10μmol/L CdCl2、50μmol/L CrCl3染毒线虫72 h后，与对照组相比，线虫平均寿命均缩短，平均寿命及生存曲线差异均有统计学意义（P均<0.05）。L4期线虫分别在2.5、50、100μmol/L上述重金属溶液中暴露72 h，世代时间延长、后代数目减少，与对照组比较差异均有统计学意义（P均<0.01）。在低浓度时，Hg生殖毒性较Pb、Cd、Cr大，As毒性较弱。结论重金属暴露对秀丽隐杆线虫寿命有一定影响，可致子代生殖能力下降。“,”Objective To evaluate the biological toxicity of heavy metals by using Caenorhabditis elegans. Methods The C. elegans at L4 stage were exposed to CdCl2 CrCl3 As2O3 PbCl2 HgCl2 with low concentrations and M9 buffer the control group for 72 h respectively and the effects of heavy metals with different concentrations on the survival time and reproduction of C. elegans were evaluated. Results After exposure to 2.5 10μmol/L HgCl2 and PbCl2 10μmol/L CdCl2 and 50μmol/L CrCl3 for 72 h respectively the life spans and survival curves of the C. elegans were different from those in the control group the differences were statistically significant all P<0.05 . After exposure to CdCl2 CrCl3 As2O3 PbCl2 and HgCl2 with the con?centrations of 2.5 50 100μmol/L for 72 h respectively the generational time and brood size of C. elegans were all different from those in the control group all P<0.01 . Among the 5 heavy metals at low concentrations the reproduction toxicity of Hg was bigger than Pb Cd Cr and the toxicity of As was the weakest. Conclusion Heavy metal exposure can affect the life span and reproductive toxicity of C. elegans.