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目的 探讨链脲佐菌素诱发的糖尿病模型大鼠的胰腺组织中自由基清除剂 SOD m RNA和磺脲类药物受体SUR m RNA的表达 ,了解糖尿病时胰腺 SOD和 SUR的关系。方法 分别在 0、4、12、2 4周取其血和胰腺 β细胞测定血糖、血脂、SOD、SUR的 m RNA表达研究 [采用逆转录 聚合酶链反应 (RT PCR) ]。结果 和正常对照组相比 ,糖尿病大鼠血糖、血脂缓慢上升 ,胰腺 β细胞的 SOD m RNA表达量明显低下 ,SUR的 m RNA也有类似改变 ,但晚于 SOD的变化 ,相关分析示二者密切相关 ,r=0 96,P<0 0 0 1。结论 2型糖尿病模型大鼠的胰腺 β细胞中存在 SOD、SUR的 m R NA表达水平低下 ,而且随着高血糖持续时间的延长 ,呈逐渐下降趋势。胰腺组织中 SOD改变可能是糖尿病时 SUR水平下降的原因之一。
Objective To investigate the expression of superoxide dismutase SOD m RNA and sulfonylurea receptor SUR m RNA in pancreas of diabetic rats induced by streptozotocin (STZ), and to investigate the relationship between SOD and SUR in diabetic pancreas. Methods The mRNA expression of blood glucose, lipids, SOD and SUR in blood and pancreatic βcells were measured at 0, 4, 12, 24 weeks respectively [RT - PCR]. Results Compared with the normal control group, the blood glucose and blood lipids in diabetic rats increased slowly, and the expression of SOD m RNA in pancreatic β cells was significantly lower. The m RNA of SUR also had similar changes, but later in the changes of SOD, the correlation analysis showed that both were close Correlation, r = 0 96, P <0 0 0 1. CONCLUSIONS: The expression of SOD and SUR mRNA in pancreatic β cells of type 2 diabetic rats is low, and gradually decreases with the duration of hyperglycemia. Changes in pancreatic tissue SOD may be one of the reasons for the decline in SUR level during diabetes.