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[目的]研究身痛逐瘀汤药理血清对大鼠诱导凋亡椎间盘髓核细胞Sox9、TGF-β1表达的影响。[方法]用细胞免疫化学和积分光密度分析等方法比较分析身痛逐瘀汤药理血清对诱导凋亡大鼠腰椎间盘髓核细胞Sox9、TGF-β1表达的影响。[结果]身痛逐瘀汤组和凋亡诱导组比较,TGF-β1明显下降,而Sox9明显上升,差异有统计学意义(P<0.01)。[结论]身痛逐瘀汤可以调控大鼠椎间盘髓核细胞内TGF-β1、Sox9表达,其延缓椎间盘退变的机制可能与调控细胞的凋亡相关因子有关。
[Objective] To investigate the effect of Shen Tong Zhu Yu Tang pharmacological serum on the expression of Sox9 and TGF-β1 in rat induced apoptosis nucleus pulposus cells. [Methods] The effects of Shen Tong Zhu Yu Tang pharmacological serum on the expression of Sox9 and TGF-β1 in lumbar disc nucleus pulposus cells of rats induced by apoptosis were comparatively analyzed by immunocytochemistry and integral optical density analysis. [Result] Compared with the apoptosis induction group, the expression of TGF-β1 in SONGTUJU decoction group was significantly decreased, while Sox9 was significantly increased, the difference was statistically significant (P <0.01). [Conclusion] Shen Tong Zhuyu Decoction can regulate the expression of TGF-β1 and Sox9 in the nucleus pulposus nucleus pulposus of rats, and the mechanism of its delaying disc degeneration may be related to the regulation of apoptosis-related factors.