建立了离子对高效液相色谱法测定米雅利桑爱儿Ａ颗粒剂中泛酸钙、维生素Ｂ６及维生素Ｂ２的含量。采用Ｃ１８柱，以含０１９％（体积分数）磷酸的００１ｍｏｌ／Ｌ庚烷磺酸钠溶液甲醇（７４∶２６）为流动相，检测波长为２２５ｎｍ。线性范围分别为：泛酸钙００６４～０３２ｍｇ／ｍｌ，维生素Ｂ６００６４～０３２ｍｇ／ｍｌ，维生素Ｂ２００１６～００８ｍｇ／ｍｌ；回收率分别为９９５％，９８２％，１００２％；精密度（ＲＳＤ）分别为０８％、０６％、０８％。 An ion-pair high performance liquid chromatography was developed for the determination of calcium pantothenate, vitamin B6 and vitamin B2 in Miyalisang Aiier A Granules. The mobile phase consisted of C18 column and 001 mol / L sodium heptane sulfonate solution with methanol containing 019% phosphoric acid (74:26). The detection wavelength was 225 nm. The linear ranges were as follows: pantothenic acid 0064 ~ 032mg / ml, vitamin B60064 ~ 032mg / ml, vitamin B20016 ~ 008mg / ml. The recoveries were 995%, 98 2% and 1002% respectively. The precision (RSD) were 08%, 06% and 08% respectively.