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α干扰素(IFN)治疗毛细胞白血病(HCL)有效,但常需长期治疗.治疗后在体内可迅速诱导生物学改变如致瘤性增强,已观察到α-IFN治疗后人类TeraⅠ及Ter Ⅱ细胞株c-ki-ras癌基因表达增强.为确定这些体外结果在体内是否也会出现,作者用Northern和Western 印迹法分析了3例HCL患者经α-IFN治疗后淋巴细胞的ki-ras RNA和蛋白质水平.用特异性ki-ras探针杂交法检测ki-ras RNA表达.用大鼠Y 13-259单克隆抗血清检测特异性蛋白.2例患者分别用重组α-IFN治疗13和12个月,每周3次,每次3×10~6U;另1例每日接受3×10~6U
Alpha-interferon (IFN) is effective in treating hairy cell leukemia (HCL), but it often requires long-term treatment. After treatment, biological changes such as tumorigenicity can be rapidly induced in vivo. Human TeraI and Ter II have been observed after α-IFN treatment. The expression of c-ki-ras oncogene is enhanced in cell lines. To determine whether these in vitro results will also occur in vivo, the authors used Northern and Western blots to analyze the ki-ras RNA of lymphocytes treated with α-IFN in 3 patients with HCL. And protein levels. Detection of ki-ras RNA expression by specific ki-ras probe hybridization. Detection of specific proteins using rat monoclonal Y 13-259 antiserum. Two patients treated with recombinant alpha-IFN 13 and 12 respectively Months, 3 times per week, 3x10~6U each time; another case receives 3x10~6U daily