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本实验主要以胚肝细胞作为细胞来源，通过贴壁处理，分离DCs的前体细胞，在含细胞因子的培养体系中培养，诱导其分化为树突状细胞。比较不同处理和不同来源细胞所得到的树突状细胞生长状况、细胞表型和异同、细胞功能，以寻找一种体外大量扩增人树突状细胞的新方法．
In this experiment, embryonic hepatocytes were used as the source of cells. DCs precursor cells were isolated by adherent treatment and cultured in culture system containing cytokines to induce their differentiation into dendritic cells. To compare the growth status, cell phenotype, similarities and differences, and cell functions of dendritic cells obtained from different treatments and cells of different origins, in order to find a new method for expanding large population of human dendritic cells in vitro.