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本文采用整体犬急性心肌缺血－再灌注模型以探讨穿心莲提取液减轻钙超负荷的机制。结扎冠脉左前降支上１／３处９０ｍｉｎ后再灌注６０ｍｉｎ较持续结扎１５０ｍｉｎ，缺血中心区心肌细胞内Ｃａ２＋增加（Ｐ＜０．０５）、Ｎａ＋明显增加（Ｐ＜０．０１），心肌细胞膜Ｃａ２＋－ＡＴＰａｓｅ活性明显降低（Ｐ＜０．０１），心肌组织ＭＤＡ含量显著增高（Ｐ＜０．０１）。而在再灌注前４５ｍｉｎ静脉给予穿心莲提取液，则见其缺血中心区心肌细胞内Ｃａ２＋降低（Ｐ＜０．０５）、Ｎａ＋明显降低（Ｐ＜０．０１），心肌细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－ＡＴＰａｓｅ活性明显增加（Ｐ＜０．０１）。心肌组织ＭＤＡ含量显著降低（Ｐ＜０．０１）。说明穿心莲提取液减轻心肌缺血－再灌注过程中钙超负荷与减轻氧自由基危害、保护心肌细胞膜ＡＴＰａｓｅ活性、降低钠超负荷有关。
In this paper, the whole canine model of acute myocardial ischemia-reperfusion was used to investigate the mechanism of andrographis paniculata extract on reducing calcium overload. Ligation of the left anterior descending coronary artery 1/90 at 90min after reperfusion 60min than continuous ligation 150min, myocardial ischemia in the central zone of intracellular Ca2 + increased (P <0.05), Na increased significantly (P <0.01), myocardial The activity of Ca2 + -ATPase in the cell membrane was significantly decreased (P <0.01), and the content of MDA in myocardial tissue was significantly increased (P <0.01). And 45 minutes before reperfusion perfusion of Andrographis paniculata extract, then the ischemic center of myocardial cell Ca2 + decreased (P <0.05), Na decreased significantly (P <0.01), myocardial cell membrane Na + -K + -ATPase , Ca2 + -ATPase activity increased significantly (P <0.01). Myocardial MDA content was significantly lower (P <0.01). It indicates that the extract of Andrographis paniculata can relieve myocardial ischemia-reperfusion process, calcium overload and reduce the harm of oxygen free radicals, protect myocardial cell membrane ATPase activity, reduce sodium overload.