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用单向火箭免疫电泳法筛选健康献血员,获得LP(a)阳性新鲜血浆。先用多价阴离子化合物NaphT—MS~(2+)沉淀包括LP(a)的全部含apoB的脂蛋白,然后超迷离心和经sepharose 6B柱、抗apo(a)IgG—Sepharose 4B免疫亲和柱柱层析,得到的LP(a)、LDL和VLDL为纯品。
One-way rocket immunoelectrophoresis screening of healthy blood donors to obtain LP (a) -positive fresh plasma. All apoB-containing lipoproteins including LP (a) were first precipitated with the multivalent anion compound NaphT-MS ~ (2+) and then ultracentrifuged and sepharose 6B-blocked, anti-apo Column chromatography gave LP (a), LDL and VLDL as pure product.