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Objective:The column chromatographic fraction of ethyl acetate(EA1,EA2,EA3,EA4 and EA5) leaf extracts of Vangueria spinosa(V.spinosa) were screened for antibacterial activity and phytochemical analysis.Methods:EA3 fraction was isolated and identified by column chromatography,thin layer chromatography,spectral data analysis and phytochemical screening were used for analysis.Results:EA3 fraction was significantly active at 4 to 64 mg/L against Staphylococcus aureus,Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa with minimum inhibitory concentration of 1.5625 to 3.1250 mg/mL.The active fraction(EA3) revealed the presence of flavonoid with retention factor value(R_f) of 0.39.The active antibacterial agent in the most potent fraction(EA3) was isolated and identified as flavonoid(-)-epicatechin-3-O-β-glucopyranoside by thin layer chromatography(TLC) and phytochemical screening.EA1 and EA2 show inhibitory activity at 4 to 64 mg/L against Staphylococcus aureus only where as fraction EA4 and EA5 do not shows any inhibitory activity within that range of concentration against any bacteria.Conclusions:The results support the ethnomedicinal use of leaf of V.spinosa for the treatment of bacterial diseases.
Objective: The column chromatographic fraction of ethyl acetate (EA1, EA2, EA3, EA4 and EA5) leaf extracts of Vangueria spinosa (V. spinosa) were screened for antibacterial activity and phytochemical analysis. Methods: EA3 fraction was isolated and identified by column chromatography , thin layer chromatography, spectral data analysis and phytochemical screening were used for analysis. Results: EA3 fraction was significantly active at 4 to 64 mg / L against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa with a minimum inhibitory concentration of 1.5625 to 3.1250 mg / mL. The active fraction (EA3) revealed the presence of flavonoid with retention factor value (R_f) of 0.39. The active antibacterial agent in the most potent fraction (EA3) was isolated and identified as flavonoid (-) - epicatechin-3 -O-β-glucopyranoside by thin layer chromatography (TLC) and phytochemical screening. EA1 and EA2 show inhibitory activity at 4 to 64 mg / L against Staphylococcus aureus only where as fraction EA4 and EA5 do not shows any inhibitory activity within that range of concentration against any bacteria. Conclusions: The results support the ethnomedicinal use of leaf of V.spinosa for the treatment of bacterial diseases.