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目的探讨新城疫病毒(NDV)和紫外线灭活的新城疫病毒(NDV-UV)在体外诱导人巨噬细胞(Mφ)对肿瘤细胞的杀伤作用。方法用淋巴细胞分离液从外周血中分离单个核细胞,经贴壁黏附法得到Mφ细胞,用NDV和NDV-UV分别诱导Mφ细胞16h作为效应细胞,Hep-2细胞作为靶细胞,然后用MTT法测定Mφ细胞杀瘤活性,并进一步比较NDV和NDV-UV对Mφ细胞杀瘤活性的影响。结果NDV和NDV-UV诱导的Mφ细胞对Hep-2细胞的杀伤率分别为(28.46±1.70)%、(22.65±2.76)%,均高于未诱导组的(6.31±0.96)%(P﹤0.01),而NDV诱导组的杀瘤率高于NDV-UV诱导组(P﹤0.01)。结论NDV和NDV-UV能在体外诱导人外周血Mφ细胞,使之杀瘤活性增强,NDV的诱导作用较NDV-UV强。
Objective To investigate the killing effect of Newcastle disease virus (NDV) and ultraviolet inactivated Newcastle disease virus (NDV-UV) on tumor cells induced by human macrophage (Mφ) in vitro. Methods Mononuclear cells were isolated from peripheral blood by lymphocyte separation liquid and Mφ cells were obtained by adherent adhesion method. Mφ cells were induced by NDV and NDV-UV for 16h respectively as effector cells and Hep-2 cells as target cells. MTT Method was used to determine the cytotoxic activity of Mφ cells. The effects of NDV and NDV-UV on the tumorigenicity of Mφ cells were further compared. Results The killing rates of Mφ cells induced by NDV and NDV-UV on Hep-2 cells were (28.46 ± 1.70)% and (22.65 ± 2.76)%, respectively, which were higher than those of non-induced cells (6.31 ± 0.96)% (P < 0.01). The killing rate of NDV-induced group was higher than NDV-UV-induced group (P <0.01). Conclusion NDV and NDV-UV can induce Mφ cells in vitro to enhance their cytotoxicity and NDV induction than NDV-UV.