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目的:观察不同剂量的糖脂消对胰岛素抵抗高血压大鼠胰岛素敏感性的影响并探讨其作用机制。方法:用高果糖饲料喂养雄性SD大鼠建立胰岛素抵抗大鼠模型,随机分为3组(每组各13只),即模型对照组、低剂量和高剂量的糖脂消组,分别喂高果糖、高果糖加不同剂量(15 g/L和60 g/L)的糖脂消。4周后,采用葡萄糖氧化酶法检测空腹血糖(FBG),用放免法测定空腹胰岛素(FINS)和瘦素(LP),用稳态模型(HOMA)评估法检测胰岛素抵抗(IR)。结果:高果糖组大鼠的LP与FINS和IR呈显著的正相关(r=0.67,P<0.05;r=0.68,P<0.05)。随着糖脂消剂量的增加,LP、FINS和IR的水平逐渐下降,但仅高剂量糖脂消组与模型对照组的差异有统计学意义(均P<0.05)。结论:糖脂消能明显改善IR,其部分机制可能与降低LP和FINS有关。
Objective: To observe the effects of different doses of glycolipid on insulin sensitivity in insulin resistance hypertensive rats and to explore its mechanism. Methods: Male SD rats were fed with high fructose diet to establish insulin resistance rat model and were randomly divided into 3 groups (13 rats in each group), namely model control group, low dose and high dose of glycolipid group, fed with high Fructose, high fructose plus different doses (15 g / L and 60 g / L) of glycolipids. After 4 weeks, fasting blood glucose (FBG) was measured by glucose oxidase method. Fasting insulin (FINS) and leptin (LP) were determined by radioimmunoassay. Insulin resistance (IR) was measured by homeostasis model assessment. Results: There was a significant positive correlation between LP and FINS and IR in high fructose group (r = 0.67, P <0.05; r = 0.68, P <0.05). The levels of LP, FINS and IR decreased gradually with the increase of glycolipid dose, but there was a significant difference between high dose glycolipid group and model control group (all P <0.05). CONCLUSION: The administration of glycolipid can significantly improve IR, and some of its mechanisms may be related to the reduction of LP and FINS.