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为明确不同基因型绿豆(Vigna radiata)萌发期耐盐碱特性,筛选耐盐碱绿豆鉴定指标,建立可靠的绿豆耐盐碱性数学评价模型,从而为绿豆抗逆栽培和耐盐碱新品种选育、推广及大规模鉴定评价提供科学依据,本研究以30个绿豆品种为试验材料,采用人工气候箱内培养皿培养,以混合盐碱(NaHCO_3与Na_2CO_3的摩尔比为9:1)模拟松嫩平原盐碱环境,在萌发期选用50 mmol·L~(-1)混合盐碱溶液胁迫处理30个绿豆品种,以蒸馏水处理为对照,测定了不同基因型绿豆在对照与胁迫处理下种子发芽率(GR)、胚芽长(GL)、胚根长(RL)、胚芽鲜重(FWG)、胚根鲜重(FWR)、胚芽干重(DWG)、胚根干重(DWR)、根冠比(RSR)、相对电导率(RC)、发芽势(GP)、发芽指数(GI)和活力指数(VI)12个生长指标,以各单项指标的耐盐碱系数作为衡量耐盐碱性的依据,利用多元分析方法对不同绿豆品种耐盐碱能力进行了综合评价。通过主成分分析将12个单项指标转化为5个相互独立的综合指标,涵盖了全部数据91.74%的信息量;利用隶属函数法和聚类分析将30个绿豆品种划分为3类;采用逐步回归建立绿豆幼苗耐盐碱性评价数学模型,D=-0.161+0.604VI+0.379GR+0.167RSR+0.156RL+0.113GL(R2=0.999),并筛选出VI、GR、RSR、RL、GL五个鉴定指标,可进行不同基因型绿豆萌发期耐盐碱性强弱的快速评价与预测。
In order to clarify the salt and alkali resistance of different genotypes of Vigna radiata in germination stage and to screen the identification index of salt-tolerant mung bean and to establish a reliable mathematic evaluation model of mung bean salt tolerance and alkalinity, Breeding, popularization and large-scale appraisal. 30 mung bean cultivars were used as test materials and cultured in Petri dishes in a climatic chamber to simulate Pinus massoniana (NaHCO 3 and Na 2 CO 3 molar ratio of 9: 1) In the tender plain saline-alkali environment, 30 mung bean cultivars were treated with 50 mmol·L -1 saline solution at the germination stage, and treated with distilled water as control. The seed germination of mung bean with different genotypes under the control and stress treatments GR, GR, RL, FWG, FWG, DWG, DWR, (RS), relative conductivity (RC), germination potential (GP), germination index (GI) and viability index (VI) Based on the comprehensive evaluation of salt-alkali tolerance of different mung bean varieties by multivariate analysis. By means of principal component analysis, 12 individual indicators were transformed into 5 mutually independent comprehensive indicators, covering 91.74% of the total data; using the membership function and clustering analysis, 30 mung bean cultivars were divided into 3 categories; The mathematic model of mung bean seedling salinity and alkalinity evaluation was established. D = -0.161 + 0.604VI + 0.379GR + 0.167RSR + 0.156RL + 0.113GL (R2 = 0.999), and five of VI, GR, RSR, RL and GL Identification of indicators, can be different genotypes mung bean germination phase of rapid evaluation and prediction of the strength of salt.