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目的 用分子技术调查中国间日疟原虫种群结构与地理分布。 方法 用滤纸血滴法采集我国 10个省 (自治区 )间日疟现症病人血样 ,用套式 、半套式 等位特异PCR基因分型法鉴定其型、族归属及其CSP基因型 ,并作流行病学统计分析。 结果 在 384个间日疟原虫分离株中 ,检出温带族 2 5 8株 ,分为 14个不同的(等位变异 )基因型 ,遍布全国各省 ,其中主带≤ 731bp的基因型仅见于南方 5省 ;热带族 79株 ,分为 5个不同基因型 ,分布于北纬 2 5°以南的 5个省 (自治区 ) ;PV 2型 16株 ,包括 2个基因型 ;另 33个分离株为不同型(族 )或不同基因型虫株的重复 (混合 )感染。 结论 目前我国北纬 2 5°以北各省是单一温带族间日疟原虫分布区 ,北纬 2 5°以南地区是温带族与热带族间日疟原虫重叠分布区 ,其中海南和云南两省局部地区同时尚存在PV 2型 ;温带族内存在地理分布明显不同的 2个基因类群。
Objective To investigate the population structure and geographical distribution of Plasmodium vivax in China using molecular techniques. Methods The blood samples of P. vivax were collected from 10 provinces (autonomous regions) in China by filter paper blood droplet method. The genotypes, genotypes and CSP genotypes were identified by nested and semi-nested allele-specific PCR genotyping For epidemiological analysis. Results Among 384 isolates of Plasmodium vivax, 258 strains of temperate zone were detected and divided into 14 different (allelic) genotypes in all provinces of China. The genotypes with the main band of ≤731bp were only found in the south 5 provinces and 79 tropical families were divided into 5 different genotypes and distributed in 5 provinces (autonomous regions) south of latitude 25 ° south. There were 16 PV 2 types including 2 genotypes and 33 isolates were Duplicate (mixed) infections of different genotypes or different genotypes. Conclusions At present, all provinces in north of latitude 25 degrees north latitude in our country are single temperate zone of P.falciparum distribution. In the area south of latitude 25 degrees north latitude, there are overlapping distribution areas of temperate and tropical P. vivax, among which Hainan and Yunnan provinces At the same time there are still PV 2 type; Temperate tribes exist in two significantly different geographical distribution of gene groups.