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目的：探讨耳蜗外淋巴液（ＰＬ）－脑脊液（ＣＳＦ）屏障存在的直接证据。方法：以内源性激素氢化可的松（ＨＣ）和外源性激素甲泼尼龙（ＭＰ）为示踪剂，应用ＨＰＬＣ技术观察示踪剂在鼓阶外淋巴液（ＰＳＴ）、前庭阶外淋巴液（ＰＳＶ）、中阶内淋巴液（ＳＭＥ）、ＣＳＦ以及血液中的动力学变化特征。结果：鼓室内注射ＨＣ１ｈ后，ＰＳＴ和ＰＳＶ中ＨＣ浓度几乎完全相同；注射后１～２ｈ，ＰＳＴ，ＰＳＶ中ＨＣ浓度同步快速降低；２～６ｈ期间，ＨＣ浓度降幅趋缓；而ＣＳＦ中ＨＣ浓度为０。鼓室内注射ＭＰ后，很快同时出现在ＰＳＴ和ＰＳＶ中，两阶ＰＬ各时间测值差异不显著；ＣＳＦ中ＭＰ为０。ＳＭＥ中ＨＣ和ＭＰ的各时间测值均显著高于ＰＳＶ，ＰＳＴ。结论：耳蜗小管不存在开放性自由通道使ＰＳＴ与ＣＳＦ直接沟通，ＣＳＦ亦不能经耳蜗小管直接进入鼓阶替换或取代ＰＬ，提示存在耳蜗ＰＬ－ＣＳＦ屏障
Objective: To investigate the direct evidence of the presence of extra-cochlear extra -mphillary (PL) -crudeal CSF barrier. Methods: The endogenous hormones hydrocortisone (HC) and exogenous hormone methylprednisolone (MP) were used as tracers. HPLC was used to observe the effect of tracer on extra-corpus epicanthora (PST), vestibular extranodal lymph PSV), mid-stage lymphatic fluid (SME), CSF, and the dynamics of the blood. Results: After intratumoral injection of HC1h, the concentration of HC in PST and PSV was almost the same. HC concentrations in PST and PSV decreased rapidly synchronously at 1 ~ 2h after injection, and decreased slowly at 2 ~ 6h. HC concentration in CSF Is 0 After intratympanic injection of MP, it appeared in both PST and PSV at the same time. There was no significant difference between two PL measurements at different time points; MP in CSF was zero. The values of HC and MP in SME were significantly higher than PSV and PST. CONCLUSIONS: There is no open free passage in the cochlear tubules to allow direct communication between PST and CSF, and CSF can not directly enter the scala tympani to replace or replace PL through the cochlear tubules, suggesting the presence of a cochlear PL-CSF barrier