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为了节省经费和使转基因模型动物品种资源得到妥善保存,该研究利用自制的梯度浓度冷冻液和解冻液结合玻璃化方式分别冷冻和解冻了非人灵长类动物的183个卵母细胞(GV期、MI期和MII期)、114个卵裂期胚胎(2-细胞期、4-细胞期和8-细胞期)及25个桑椹期胚胎。其中食蟹猴卵母细胞67个,卵裂期胚胎45个,桑椹期胚胎11个;恒河猴卵母细胞116个,卵裂期胚胎69个,桑椹期胚胎14个。复苏后存活率分别为56/67(83.58%)、36/45(80.00%)、9/11(81.82%)、102/116(87.93%)、55/69(79.71%)和11/14(78.57%)。结果表明,快速玻璃化冷冻法简便且胚胎存活率高,是一种较好的冷冻食蟹猴和恒河猴卵母细胞及胚胎的方法。
In order to save money and conserve the genetic resources of genetically modified animals, 183 oocytes of non-human primate were frozen and thawed by self-made gradient concentration thawing solution and thawing solution combined with vitrification (GV stage , MI and MII), 114 cleavage stage embryos (2-cell stage, 4-cell stage and 8-cell stage) and 25 mulberry stage embryos. Among them, there were 67 cynomolgus monkey oocytes, 45 cleavage stage embryos, 11 morula stage embryos, 116 rhesus monkey oocytes, 69 cleavage stage embryos and 14 morula stage embryos. The survival rates after resuscitation were 56/67 (83.58%), 36/45 (80.00%), 9/11 (81.82%), 102/116 (87.93%), 55/69 (79.71%) and 11/14 78.57%). The results showed that the rapid vitrification method is simple and has high embryo survival rate, which is a better method for freezing cynomolgus and rhesus oocytes and embryos.