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目的了解男男性接触者(MSM)HIV-1感染者毒株亚型类型,分析HIV-1在该人群中传播的危险因素。方法收集石家庄市2006至2008年报告的51例HIV-1抗体阳性的MSM的血液样本,提取前病毒DNA,利用套氏PCR方法扩增包膜蛋白env基因部分片断,并对扩增片断进行序列测定和分析。结果32份毒株为B亚型,组内基因离散率为(13.3±0.8)%,与标准株B.NL.0067的基因离散率为(16.0±1.6)%;16份毒株为CRF01-AE亚型,组内基因离散率为(7.1±0.7)%,与标准株CRF01-AE.TH.90的基因距离为(11.0±1.5)%;3份毒株为CRF07-BC亚型,组内基因离散率为(9.0±1.2)%,与标准株07-BC.CN.97的基因离散率为(9.3±1.3)%。结论HIV-1在石家庄市MSM中的流行株以B亚型和重组亚型CRF01-AE最常见,也存在CRF07-BC重组亚型。
Objective To understand the subtypes of HIV-1-infected MSM and to analyze the risk factors for HIV-1 transmission in this population. Methods Fifty-one HIV-1 positive MSM blood samples were collected from 2006 to 2008 in Shijiazhuang City, and the proviral DNA was extracted. The envelope protein env gene fragment was amplified by PCR, and the amplified fragment was sequenced Determination and analysis. Results The results showed that 32 strains were subtype B with a gene transfer rate of (13.3 ± 0.8)% in the group and (16.0 ± 1.6)% with the standard strain B.NL.0067. Sixteen strains were CRF01- AE subtypes. The gene isolation rate was (7.1 ± 0.7)% within the group and (11.0 ± 1.5)% to the standard strain CRF01-AE.TH.90. The three strains were CRF07-BC subtype The internal gene dispersion rate was (9.0 ± 1.2)%, and that of the standard strain 07-BC.CN.97 was (9.3 ± 1.3)%. Conclusion The prevalence of HIV-1 in MSM in Shijiazhuang City is the most common in subtypes B and CRF01-AE, and the CRF07-BC subtype is also present.