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目的:比较铜绿假单胞菌(PA)注射液与5-Fu腹腔化疗对大鼠结肠吻合口愈合的影响并探讨其机制。方法:将40只SD大鼠随机均分为4组,即模型对照组、铜绿假单胞菌注射液皮下注射组(PA皮下注射组)、PA注射液皮下注射+术中腹腔喷洒组(皮下注射+腹腔喷洒组)、5-Fu腹腔化疗组(5-Fu组),每组10只,均行结肠部分切除再吻合术。术后7 d检测吻合口的破裂压、病理学形态、胶原纤维含量、转化生长因子(TGF-β)及纤维连接蛋白(FN)表达。结果:PA皮下注射组和PA皮下注射+腹腔喷洒组脏器粘连程度与模型对照组大鼠相近,吻合口破裂压、炎性细胞数量、胶原纤维沉积及TGF-β和FN表达显著高于模型对照组和5-Fu组。结论:PA注射液可提高机体免疫状态,增加吻合口炎性细胞数量,促进结肠吻合口早期愈合,降低吻合口漏的发生,而且不会加重粘连程度。
Objective: To compare the effects of Pseudomonas aeruginosa (PA) injection and 5-Fu intraperitoneal chemotherapy on the healing of colonic anastomosis in rats and its mechanism. Methods: Forty SD rats were randomly divided into four groups: model control group, Pseudomonas aeruginosa injection subcutaneous injection group (PA subcutaneous injection group), PA injection subcutaneous injection + intraperitoneal injection group (subcutaneous Injection + intraperitoneal injection group), 5-Fu intraperitoneal chemotherapy group (5-Fu group), with 10 in each group. All patients underwent partial resection and anastomosis. At 7 days after operation, the ankle rupture pressure, pathological morphology, collagen fiber content, transforming growth factor (TGF-β) and fibronectin (FN) expression were detected. Results: The degree of adhesions in PA subcutaneous injection group and intraperitoneal injection group was similar to that in model control group. The ankle rupture pressure, the number of inflammatory cells, the deposition of collagen fibers and the expression of TGF-β and FN in model group were significantly higher than those in model control group Control group and 5-Fu group. Conclusion: PA injection can improve immune status, increase the number of anastomotic inflammatory cells, promote early healing of colonic anastomosis and reduce the incidence of anastomotic leakage, and will not aggravate the degree of adhesion.