将油桐尺蠖（Ｂｕｚｕｒａｓｕｐｐｒｅｓｓａｒｉａ）核多角体病毒晚期基因──多角体蛋白基因启动子及５′端编码区，以两种不同方式置于缺乏启动子的氯霉素乙酰基转移酶（ＣＡＴ）基因上游，使其分别终止在不同翻译终止位点，其宿主菌具有明显不同的氯霉素抗性，最高达２００ｍｇ／Ｌ　ＬＢ培养基以上，表明昆虫病毒启动子能启动原核基因表达。对多角体蛋白基因启动子能在大肠杆菌中有效工作的原因进行了讨论。 The late stage polyhedrosis virus gene, Polyhedrin gene promoter and 5 ’end coding region of Buzurasuppressaria, was placed in two different ways on a chloramphenicol acetyltransferase (CAT) gene lacking the promoter Upstream, they were terminated at different translation termination sites, respectively, and their host bacteria had significantly different resistance to chloramphenicol up to 200mg / L LB medium, indicating that the insect virus promoter can initiate prokaryotic gene expression. The reasons why polyhedrin gene promoters work well in E. coli are discussed.