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目的探讨牛磺酸对铝染毒大鼠肝脏抗脂质过氧化功能和肝功能的改善效果。方法将42只健康SPF级雄性Wistar大鼠按体重随机分为6组,分别为阴性对照(生理盐水)组、铝染毒组和低、中、高剂量牛磺酸干预组及牛磺酸预防组,每组7只。每天灌胃1次,每周5 d。前4周铝染毒组和低、中、高剂量牛磺酸干预组及牛磺酸预防组染毒281.40 mg/kg三氯化铝,其中,牛磺酸预防组染毒4 h后给予400 mg/kg牛磺酸;后4周,铝染毒组给予1 ml生理盐水,低、中、高剂量牛磺酸干预组和牛磺酸预防组分别灌胃200、400、800、400 mg/kg牛磺酸。测定肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力以及血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活力和总胆汁酸(TBA)含量。结果与铝染毒组相比,高剂量牛磺酸干预组、牛磺酸预防组肝组织中SOD活力、GSH-Px活力升高,肝组织MDA含量和血清中ALT、AST活力、TBA含量降低;且与阴性对照组相比无明显变化。结论牛磺酸在一定剂量范围内对铝染毒大鼠肝脏抗氧化系统和肝脏功能有改善效果,可以有效地减轻铝造成的肝脏损伤。
Objective To investigate the effect of taurine on anti-lipid peroxidation and liver function in liver of aluminum-exposed rats. Methods Forty-two healthy SPF male Wistar rats were randomly divided into 6 groups according to body weight: negative control (saline) group, aluminum exposure group and low, medium and high doses of taurine intervention group and taurine prevention Group, 7 in each group. Gavage 1 times a day, 5 days a week. In the first 4 weeks of exposure to aluminum and low, medium and high doses of taurine intervention group and taurine prevention group exposure 281.40 mg / kg of aluminum trichloride, which taurine prevention group were given 4 h after 400 mg / kg taurine. After 4 weeks, 1 ml saline was given to the aluminum exposure group, while the low, medium and high doses taurine intervention group and the taurine prevention group were administered orally with 200, 400, 800 and 400 mg / kg Taurine. The contents of malondialdehyde (MDA) and activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and serum alanine aminotransferase (ALT) Aminotransferase (AST) activity and total bile acid (TBA) content. Results Compared with the aluminum-exposed group, the activity of SOD and the activity of GSH-Px in liver tissue of high-dose taurine-induced and taurine-preventive groups were increased, the content of liver MDA and the activities of ALT, AST and TBA in serum were decreased ; And no significant change compared with the negative control group. Conclusion Taurine can improve the liver anti-oxidant system and liver function of aluminum-exposed rats in a certain dose range, which can effectively reduce the liver damage caused by aluminum.