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肠上皮内淋巴细胞 (iIEL)是具有特殊表型和发育分化机制的淋巴细胞 ,在消化道抗感染免疫和经口免疫耐受中起重要作用。本文参考国外多种方法 ,对以往采用的iIEL分离纯化技术进行改进 ,并应用流式细胞仪和形态学检查对分离纯化后的iIEL进行鉴定。结果显示 ,每只鼠可获得 4× 1 0 6～ 1 0× 1 0 6个iIEL ,iIEL中CD3+T细胞的含量为 87 8%。本文建立了一种简便、高效、稳定的iIEL提取方法
Intestinal intraepithelial lymphocytes (iIEL) are lymphocytes that have a special phenotype and developmental differentiation mechanism and play an important role in the anti-infective and oral immunological tolerance of the digestive tract. This article refers to a variety of foreign methods, the iIEL separation and purification techniques used in the past to improve, and the use of flow cytometry and morphological examination of isolated and purified iIEL identification. The results showed that 4 × 106 ~ 106 × 106 iIELs were obtained from each mouse, and the percentage of CD3 + T cells in iIEL was 87.8%. In this paper, a simple, efficient and stable iIEL extraction method is established