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在形态学分类的基础上,通过16S~23S rRNA ISR(Intergenic spacer regions)序列分析,对樟树内生细菌EBS05进行了分类鉴定,结果表明EBS05为枯草芽孢杆菌。测定了EBS05代谢活性物质的理化性质,结果表明,活性组分在λ213.5nm处有最大光吸收峰;在pH5~8范围内其抗菌活性稳定,当pH<4.0或pH>9.0时,抗菌活性显著降低;热稳定性良好,60°C~80°C处理2h后,抗菌活性不变,1×105Pa灭菌30min后,抗菌活性仍然保持在65%以上;具有较强的抗紫外线照射能力,对蛋白酶K不敏感;具有较好的醇溶性,易溶于甲醇和乙醇,不溶于乙酸乙酯、乙腈和石油醚等有机溶剂。
Based on the morphological classification, the endophytic bacteria EBS05 was identified by 16S ~ 23S rRNA ISR sequence analysis. The results showed that EBS05 was Bacillus subtilis. The physicochemical properties of the active metabolites of EBS05 were determined. The results showed that the active components had the maximum light absorption peak at λ213.5nm. The antibacterial activity of EBS05 was stable at pH5-8. When the pH was less than 4.0 or pH 9.0, the antibacterial activity Significantly reduced; good thermal stability, 60h C ~ 80 ° C treatment 2h, the antibacterial activity unchanged, 1 x 105Pa sterilization 30min, the antibacterial activity remained at 65% or more; has a strong ability to resist ultraviolet radiation, Not sensitive to proteinase K; Has good alcohol solubility, soluble in methanol and ethanol, insoluble in ethyl acetate, acetonitrile and petroleum ether and other organic solvents.