论文部分内容阅读
目的和方法 :应用ＳＰＤＰ加抗低分化鼻咽癌上皮细胞表面相关抗原的单抗ＢＡＣ5 与中华眼镜蛇膜毒素(ＣＴ)偶联 ,制备免疫毒素 (ＩＴ)。并用放射性核素双标记、示综、免疫显像和电镜等技术 ,实验观察ＩＴ在体内外对鼻咽癌作用及探讨其作用机制。结果 :ＢＡＣ5 在肿瘤组织部位呈
PURPOSE AND METHODS: Immunotoxin (IT) was prepared by the conjugation of SPDP with monoclonal antibody BAC5 against surface antigen of epithelial cell of poorly differentiated nasopharyngeal carcinoma and Chinese cobra membrane toxin (CT). The effects of IT on nasopharyngeal carcinoma (NPC) in vitro and in vivo were investigated experimentally with double labeling of radionuclides, immunoblotting and electron microscopy. Results: BAC5 was found in the tumor tissue