论文部分内容阅读
目的 :观察乙肝病毒 (HBV)感染者血清的一氧化氮 (NO)、一氧化氮合酶 (NOS)水平 ,探讨和研究NO在HBV感染过程中的变化和作用。方法 :应用ELISA法检测HBV血清标志物 ,并将HBV阳性血清分为五个常见模式组 ,应用Griess法测定各组血清NO和NOS水平与正常对照。结果 :HBsAg( +)、抗HBc( +)组 (第 4组 )和HBsAg( +)、HBeAg( +)、抗HBc( +)组 (第 5组 )显著降低 ;抗HBs( +)、抗HBc( +)组 (第 2组 )和HBsAg( +)、抗HBe( +)、抗HBc( +)组 (第 3组 ) ,NO和NOS回升 ;抗HBs( +)组 (第 1组 )和对照组水平相近。结论 :HBV在体内复制活跃时 ,可能抑制NOS活性 ,使NO合成减少 ,同时由于NO发挥对肝细胞的保护作用而使消耗增加。检测血清NO、NOS可观察HBV感染程度和预后。NO用于HBV感染的治疗有待研究
Objective: To observe the levels of serum nitric oxide (NO) and nitric oxide synthase (NOS) in patients with hepatitis B virus (HBV) infection, and to explore and study the changes and effects of NO in HBV infection. Methods: Serum HBV markers were detected by ELISA. HBV positive sera were divided into five groups. Griess method was used to determine the serum levels of NO and NOS in each group and the normal controls. Results: The levels of HBsAg (+), anti HBs (group 4) and HBsAg (+), HBeAg (+) and anti HBc (group 5) HBsAg (+), anti-HBe (+) and anti-HBc (group 3) Similar to the control group. CONCLUSIONS: When HBV is actively replicated in vivo, it may inhibit the activity of NOS and decrease the synthesis of NO. At the same time, consumption of HBV increases due to its protective effect on hepatocytes. Serum NO, NOS can be observed HBV infection and prognosis. The use of NO for the treatment of HBV infection remains to be seen