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目的初步了解深圳地区引起临床婴幼儿急性腹泻中常见病毒的病原分布情况,为临床防治提供依据。方法采用多重RT-PCR方法检测婴幼儿急性腹泻常见病毒:轮状病毒(RV)、星状病毒(AstV)、诺如病毒(NV),采用单重PCR法筛查临床腹泻标本肠道腺病毒(EAdV)。结果在440份标本中,共检出197例至少感染一种腹泻病毒,总阳性率为44.77%(197/440),其中183例为一种病毒感染,14例(3.18%)为混合感染。4种腹泻病毒以RV感染为主,阳性率为28.64%(126/440);其次是EAdV,阳性率为8.86%(39/440);NV阳性率为5.45%(24/440);AstV阳性率为1.82%(8/440)。混合感染以RV合并其他病毒感染为主,占92.86%。结论 RV、EAdV和NV是引起深圳地区婴幼儿急性腹泻的主要病毒致病原,混合感染以RV联合其他病毒感染为主,临床上应根据所感染病原体制定有针对性的诊治措施。
Objective To understand the distribution of common pathogens in acute diarrhea in infants and young children in Shenzhen and provide the basis for clinical prevention and treatment. Methods The common diarrhea virus (RV), astrovirus (NV) and norovirus (NV) were detected by multiplex RT-PCR in children and adolescents. (EAdV). Results Of the 440 samples, 197 were detected as at least one diarrhea virus with a total positive rate of 44.77% (197/440). Among them, 183 were virus-infected and 14 (3.18%) were mixed infections. Four kinds of diarrhea virus were mainly infected with RV, the positive rate was 28.64% (126/440); followed by EAdV, the positive rate was 8.86% (39/440); the positive rate of NV was 5.45% (24/440) The rate was 1.82% (8/440). Mixed infection with RV infection and other virus-based, accounting for 92.86%. Conclusions RV, EAdV and NV are the major causative pathogens causing acute diarrhea in infants and young children in Shenzhen. The combined infection is mainly associated with RV and other viral infections. Clinically, specific diagnosis and treatment measures should be formulated according to the pathogens infected.