论文部分内容阅读
目的研究皮肤鳞状细胞癌(SCC)和基底细胞癌(BCC)中缺氧诱导因子-1α(HIF-1α)的表达,分析肿瘤组织中HIF-1α与血管生成的相关性。方法采用免疫组化S-P法检测石蜡包埋组织(其中SCC 25例,BCC 25例,正常皮肤20例)中HIF-1α的表达,并用CD34标记血管内皮细胞,计算微血管密度(MVD)。结果HIF-1α主要表达于瘤细胞胞浆中,在两种肿瘤组织中均呈弥漫性表达;CD34表达于血管内皮细胞胞膜,分布于间质及癌巢。HIF-1α在SCC和BCC中表达阳性率分别为88%(22/25)、56%(14/25),SCC与BCC比较有显著性差异(P<0.05)。HIF-1α在正常皮肤中阴性表达。MVD平均值在SCC和BCC中分别为42.55±5.02,23.47±6.21,两者比较有显著性差异(P<0.05)。HIF-1α阳性的肿瘤组织MVD高于其表达阴性者(P<0.05)。结论HIF-1α在两种肿瘤组织尤其是SCC中过表达,HIF-1α可能通过促进肿瘤新生血管形成,从而促进SCC和BCC的生长、浸润和转移。
Objective To investigate the expression of hypoxia inducible factor-1α (HIF-1α) in cutaneous squamous cell carcinoma (SCC) and basal cell carcinoma (BCC), and to analyze the correlation between HIF-1α and angiogenesis. Methods The expression of HIF-1α in paraffin-embedded tissue (25 cases of SCC, 25 cases of BCC and 20 cases of normal skin) was detected by immunohistochemical S-P method. Microvessel density (MVD) was calculated by labeling CD34 with vascular endothelial cells. Results HIF-1α was mainly expressed in the cytoplasm of tumor cells and diffusely expressed in both tumor tissues. CD34 expression was found in the membrane of vascular endothelial cells and distributed in the stroma and cancer nests. The positive rates of HIF-1α expression in SCC and BCC were 88% (22/25) and 56% (14/25), respectively. There was a significant difference between SCC and BCC (P <0.05). HIF-1α is negatively expressed in normal skin. The mean MVD was 42.55 ± 5.02 and 23.47 ± 6.21 in SCC and BCC, respectively, with significant difference (P <0.05). MVD in HIF-1α-positive tumors was higher than that in HIF-1α-positive tumors (P <0.05). Conclusion HIF-1α is overexpressed in two kinds of tumor tissues, especially in SCC. HIF-1α may promote the growth, invasion and metastasis of SCC and BCC by promoting angiogenesis.