提出了分光光度法测定蛋白质的新方法.在酸性介质中,蛋白质能和溴连苯三酚红结合成为超分子化合物,用硝化纤维素滤膜富集蛋白质-溴连苯三酚红的复合物,该体系及滤膜可溶解于酸化了的二甲亚砜中形成稳定的有色溶液,其最大吸收波长为425nm.当富集倍数为50时,本方法的检测限为7μg/L,蛋白质在5mL溶剂中的含量为0～350μg时符合朗伯-比尔定律.操作简单,用于样品中蛋白质的测定,结果满意. A new method for the spectrophotometric determination of protein was proposed.In proteinase, the protein could combine with bromopyrogallol red to form a supramolecular compound, and the protein-bromopyrogallol red complex was enriched with nitrocellulose filter , The system and the filter can be dissolved in acidified dimethyl sulfoxide to form a stable colored solution with a maximum absorption wavelength of 425 nm. When the enrichment factor is 50, the detection limit of this method is 7 μg / L, The results of Lambert-Beer’s law are in accordance with Lambert-Beer’s law when the content of 0-550μg in 5mL solvent is used for the determination of protein in the sample with satisfactory results.