论文部分内容阅读
目的:研究丹参多酚酸盐预防大鼠术后肠粘连的作用并探讨其机制。方法:雄性SD大鼠50只,随机分为5组:正常对照组、模型组和丹参多酚酸盐低、中、高剂量组。除正常对照组外,其余大鼠均制备肠粘连模型。正常对照组和模型组每天腹腔注射葡萄糖;丹参多酚酸盐低、中、高剂量组每天腹腔注射丹参多酚酸盐,连续注射8d。各组于术后第8天取血,肉眼观察肠粘连的程度分级;取盲肠与腹壁粘连组织做病理观察;酶联免疫吸附法测定细胞因子白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-4(interleukin-4,IL-4)及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平。结果:丹参多酚酸盐能显著减轻大鼠的肠粘连程度,明显降低肠粘连大鼠IL-1β和TNF-α的表达水平,抑制纤维结缔组织的增生,但对IL-4的表达水平几乎没有影响。结论:丹参多酚酸盐可减轻大鼠肠粘连程度,降低IL-1β和TNF-α的表达水平,抑制纤维结缔组织的增生,这可能是其预防肠粘连的机制。
Objective: To study the effect of salvianolate on postoperative intestinal adhesion in rats and its mechanism. METHODS: Fifty male Sprague-Dawley rats were randomly divided into 5 groups: normal control group, model group, and low, medium and high doses of Salvianolate. In addition to the normal control group, all the other rats were prepared intestinal adhesion model. The normal control group and the model group were injected with glucose daily; Danshen polyphenols low-, middle-, and high-dose groups were injected intraperitoneally with Salvia polyphenolate for 8 days. Blood samples were collected on the 8th day after operation. The extent of intestinal adhesion was visually observed. The adhesion between the cecum and abdominal wall was taken for pathological examination. The interleukin-1β (IL-1β) was determined by enzyme-linked immunosorbent assay. ), Interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) levels. Results: Danshen polyphenolate can significantly reduce the extent of intestinal adhesion in rats, significantly reduce the expression of IL-1β and TNF-α in rats with intestinal adhesion, and inhibit the proliferation of fibrous connective tissue, but the expression level of IL-4 is almost the same. No effect. Conclusion: Danshen polyphenolate can reduce the degree of intestinal adhesion in rats, reduce the expression of IL-1β and TNF-α, and inhibit the proliferation of fibrous connective tissue, which may be the mechanism of prevention of intestinal adhesion.