论文部分内容阅读
脂质体作为药物口服载体,面临的主要障碍之一是其在肠道胆汁盐和肠道酶的作用下极不稳定,造成包裹药物的损失和破坏。研究中,应用丙酮沉淀、薄层制备层析以及甲醇沉淀相结合的方法,从培养的古细菌Sulfolobus acidocaldarius中分离和纯化极性脂质抽提片段(polarlipid fraction extract,PLFE),制备了由PLFE为组分的四醚脂质体;并着重评估其在模拟肠液中的稳定性。结果显示:改进的提取方法操作简单、有效;在模拟人肠道环境的体外稳定性实验中,四醚脂质体的稳定性明显优于普通磷脂脂质体。这些结论表明,具有独特结构的极性脂质组成的四醚脂质体,具备作为药物的口服输送载体的潜力。
One of the major obstacles to liposomes as oral carriers of drugs is their extreme instability under the action of intestinal bile salts and intestinal enzymes, resulting in the loss and destruction of entrapped drugs. In this study, the polar lipid fraction extract (PLFE) was isolated and purified from the cultured Archaea Sulfolobus acidocaldarius by a combination of acetone precipitation, TLC and methanol precipitation. As a component of tetraether liposomes; and its evaluation in the simulated intestinal fluid stability. The results showed that the improved extraction method was simple and effective. The stability of the tetraether liposomes was significantly better than that of the common phospholipid liposomes in simulating the in vitro stability of human intestinal environment. These findings indicate that the tetraethelfoliposomes of polar lipids with a unique structure possess the potential of being an orally administered carrier for drugs.