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目的 :建立了血浆中赖氨匹林浓度的 HPL C测定方法并用于其制剂的生物利用度研究。方法 :血浆样品先水解处理使赖氨匹林全部转化为水杨酸 ,水解后的血浆样品用乙酸乙酯萃取 ,在μ- Bondapark ODS柱上分析 ,流动相为磷酸盐缓冲液 (p H2 .0 ) -甲醇 (6 5∶ 35 ) ,非那西丁做内标 ,在紫外 30 5 nm处检测。结果 :水杨酸在 1～ 10 0μg· ml- 1范围内 ,峰面积与其浓度呈良好线性关系 (r=0 .9999) ,其最低检测限为 0 .33μg· ml- 1 ,回收率为 10 4%～ 112 % ,日内 RSD为 1.8%～ 4.0 % ,日间 RSD为 4.2 %～ 6 .8%。结论 :该方法简易、准确 ,适合于赖氨匹林制剂的药物动力学和生物利用度研究
OBJECTIVE: To establish a HPLC method for the determination of plasma lysipatulin in plasma and to study its bioavailability. METHODS: The plasma samples were first hydrolyzed to convert all the paracetamol to salicylic acid. The hydrolyzed plasma samples were extracted with ethyl acetate and analyzed on a μ-Bondapark ODS column using a mobile phase of phosphate buffer (p H2. 0) -methanol (6:35), phenacetin as internal standard, and detected at UV 30 5 nm. Results: There was a good linear relationship between the peak area and the concentration of salicylic acid (r = 0.9999) in the range of 1 ~ 100μg · ml-1, and the minimum detection limit was 0.33μg · ml-1. The recovery rate was 10 4% ~ 112%, intraday RSD was 1.8% ~ 4.0%, daytime RSD was 4.2% ~ 6.8%. Conclusion: The method is simple, accurate and suitable for the study of pharmacokinetics and bioavailability of lysipirin