用细胞培养方法和大鼠脑缺血损伤模型，研究乙酰基蝙蝠葛苏林碱（Ｏ，Ｏａｃｅｔｙｌｄａｕｒｉｓｏｌｉｎｅ，Ａｄａｕ）对缺血性脑损伤的保护作用。结果表明，Ａｄａｕ对低钾、高钾、ＢａｙＫ８６４４及去甲肾上腺素所引起ＰＣ１２细胞内游离钙浓度增加有抑制作用，其ＩＣ５０分别为４６３±０７２，０１５±００２，９３７±１７６和１００±１４μｍｏｌ·Ｌ－１。Ａｄａｕ在整体大鼠双动脉结扎（２５，５，１０ｍｇ·ｋｇ－１，ｉｖ）和四动脉结扎（５，１０ｍｇ·ｋｇ－１，ｉｖ）模型上，均能降低缺血脑组织脂质过氧化物含量，增加超氧物歧化酶（ＳＯＤ）活性。提示Ａｄａｕ对脑缺血损伤有明显的保护作用。 To study the protective effect of acetyl-daurisurine (Ado) on ischemic brain injury by cell culture and rat cerebral ischemia model. The results showed that Adau inhibited the increase of intracellular free calcium concentration in PC12 cells induced by hypokalemia, hyperkalemia, BayK8644 and norepinephrine, with IC50 values ​​of 463 ± 072 and 015 ± 002, respectively. 937 ± 176 and 100 ± 14μmol·L-1. Adau decreased the content of lipid in ischemic brain tissue in the whole rat model of double arteries ligation (2.5, 5, 10 mg · kg -1, iv) and tetanic artery ligation (5 and 10 mg · kg -1, iv) Peroxide content, increase superoxide dismutase (SOD) activity. Tip Adau obvious protective effect on cerebral ischemia injury.