论文部分内容阅读
采用基因枪法将苏云金杆菌(简称Bt)的杀虫毒蛋白基因(CryIA)导入东北春玉米自交系铁7922的幼胚中,诱导愈伤组织。抗虫基因的表达载体是pBI121,包含Bt杀虫毒蛋白基因和新霉素磷酸转移酶(NPTII)基因,筛选标记bar由CaMV35S启动子驱动。通过对后代植株的除草剂草丁膦(PPT)筛选、分子鉴定和ELISA法检测,证明外源基因已经整合到玉米基因组中并可以稳定遗传。对转基因株系进行田间接玉米螟虫卵,调查不同生长时期的危害指数,最终筛选出一批抗玉米螟自交系。
The insecticidal toxin gene (CryIA) of Bacillus thuringiensis (Bt) was introduced into immature embryos of the iron maize inbred line Northeast China by gene gun method to induce callus. The expression vector for the insect-resistant gene is pBI121, which contains the Bt insecticidal toxin gene and the neomycin phosphotransferase (NPTII) gene and the selection marker bar is driven by the CaMV35S promoter. The herbicide glufosinate (PPT) screening, molecular identification and ELISA assay of progeny plants proved that the foreign gene has been integrated into the maize genome and can be stably inherited. The transgenic lines were maize borer eggs in the field, to investigate the different growth stages of the hazard index, and finally screened a number of anti-corn borer inbred lines.