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目的研究民族药披针新月蕨Abacopteris penangiana化学成分并评价其细胞毒活性。方法以硅胶柱、凝胶柱色谱分离,制备HPLC纯化,采用MS,1、2 D-NMR等波谱方法进行结构鉴定,MTT法测试各化合物的细胞毒活性。结果从披针新月蕨中分离鉴定了6个化合物,分别为4(S),5,7-三羟基-4′-甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷[4(S),5,7-trihydroxy-4′-methoxy-6,8-di methyl-2(R)-flavan-5,7-O-β-D-diglucopyrano-side,eruberin B,1];5,7-二羟基-4(S),4′-二甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷[5,7-di-hydroxy-4(S),4′-di methoxy-6,8-di methyl-2(R)-flavan-5,7-O-β-D-diglucopyranoside,eruberin C,2];5,7-二羟基-4′-甲氧基-6-羟甲基-8-甲基-2″,4(S)-氧-2(R)-黄烷-5-O-β-D-葡萄糖苷[5,7-dihydroxy-4′-methoxy-6-hydroxymethyl-8-methyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopyranoside,3];5,7-二羟基-4′-甲氧基-6,8-二甲基-2″,4(S)-氧-2(R)-黄烷-5-β-D-葡萄糖苷[5,7-dihydroxy-4′-methoxy-6,8-di methyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopyranoside,eruberin A,4];高圣草素[5,7,4′-trihydroxy-3′-methoxyflavanone,homoeriodictyol,5];对-甲氧基桂皮醛(p-methoxycinnamaldehyde,6)。化合物3、4对L929及HeLa细胞的IC50值分别约为13.05、24.75μg/mL,25.11、18.32μg/mL。结论化合物3为新的天然产物,化合物2、5、6首次从新月蕨属植物中分得,化合物3、4对L929和HeLa具有显著抑制活性。
Objective To study the chemical constituents of Abacopteris penangiana and evaluate its cytotoxic activity. Methods The compounds were isolated by silica gel column, gel column chromatography and preparative HPLC. Their structures were identified by MS, 1, 2 D-NMR and other spectral methods. The cytotoxicity of each compound was tested by MTT assay. Results Six compounds were isolated and identified from the new fern of Phylloscopoietin, which were 4 (S), 5,7-trihydroxy-4’-methoxy-6,8-dimethyl-2 (R) 5,7-O-β-D-glucoside [4 (S), 5,7-trihydroxy-4’-methoxy-6,8-di methyl-2 (R) O-β-D-diglucopyrano-side, eruberin B, 1] 5,7-dihydroxy-4 (S), 4’-dimethoxy-6,8-dimethyl-2 (R) (5,7-di-hydroxy-4 (S), 4’-di methoxy-6,8-di methyl-2 (R) -flavan-5 , 7-O-β-D-diglucopyranoside, eruberin C, 2]; 5,7- dihydroxy- 4’- methoxy- 6-hydroxymethyl-8-methyl- 5-O-β-D-glucoside [5,7-dihydroxy-4’-methoxy-6-hydroxymethyl- 2 (R) -flavan-5-O-β-D-glucopyranoside, 3]; 5,7-dihydroxy-4’-methoxy-6,8-dimethyl- (R) -flavan-5-β-D-glucoside [5,7-dihydroxy-4’-methoxy-6,8-di methyl- ) -flavan-5-O-β-D-glucopyranoside, eruberin A, 4]; 5,7,4’-trihydroxy-3’-methoxyflavanone (homoeriodictyol, Aldehyde (p-methoxycinnamaldehyde, 6). The IC50 values of compound 3,4 for L929 and HeLa cells were about 13.05, 24.75μg / mL, 25.11 and 18.32μg / mL, respectively. Conclusion Compound 3 is a new natural product. Compounds 2, 5, and 6 were isolated from Pteridophyta for the first time. Compounds 3 and 4 showed significant inhibitory activity against L929 and HeLa.