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目的:探讨电针胃经穴的大鼠血清对胃黏膜细胞表皮生长因子受体(EGFR)信号转导通路的影响.方法:60只大鼠随机分为正常组、胃经组、胆经组、胃经+PD153035组和胆经+PD153035组,采用水浸束缚法制作胃黏膜损伤大鼠模型,链霉蛋白酶消化法分离胃黏膜细胞,分别用EGFR抑制剂PD153035和血清孵育胃黏膜细胞,应用酶联免疫吸附法检测PLCγ-1活性,同位素掺入法检测蛋白激酶C(PKC)活性,逆转录聚合酶链反应法检测c-myc基因的表达水平.结果:模型组大鼠胃黏膜细胞PLCγ-1、PKC和c-myc基因有微弱表达;胃经组和胆经组大鼠胃黏膜细胞PLCγ-1、PKC和c-myc基因呈现较强表达,其中胃经组大鼠表达最强烈,两组相比,差异有统计学意义(P“,”Objective To study the effect of the serum derived from the rats treated with electroacupunctre at Stomach Meridian acupoints on the EGFR signal transduction pathway in gastric mucosal cells. Methods Sixty rats were randomly divided into 5 groups:model group,Stomach (ST) Meridian group,Gallbladder (GB) Meridian group,ST+PD153035 group and GB+PD153035 group. Water-immersion and restrained stress method was adopted for preparation of the stress-induced gastric ulcer rat model,and the gastric mucosa cells were separated by using pronase digestion method. Gastric mucosa cells were incubated respectively with PD153035,EGFR inhibitor and the serum; PLCγ-1's expression level in gastric mucosa cells was tested by enzyme-linked immunosorbant assay (ELISA),and PKC activity by isotope mingling method; and reverse transcription polymerase chain reaction assay (RT-PCR) was used for testing expression level of c-myc gene. Results The expression levels of PLCγ-1,PKC and c-myc gene were lower in gastric mucosa cells in the model group; there were relatively high expression levels of PLCγ-1,PKC and c-myc gene in the ST Meridian group and the GB Meridian group,with the highest expression levels of PLCγ-1,PKC and c-myc gene in the ST Meridian,and with a significant difference between the ST Meridian group and the GB Meridian group (P<0.01); and relative lower expression levels of PLCγ-1,PKC and c-myc gene were seen in the ST + PD153035 group and the GB+ PD153035 group; and there was a significant difference between the ST Meridian group and the ST + PD153035 group (P<0.01). Conclusion There was a close correlation between the healing effect of electroacupuncture and activation of the EGFR signal transduction pathway in the impaired gastric mucosa cells,which was also one proof for the TCM theory:“There is relative specificity between the meridians and viscera”.