论文部分内容阅读
目的 探讨血小板衍生生长因子BB(PDGF-BB)在慢性乙型肝炎患者肝纤维化发病机制中的作用。 方法 对43例慢性乙型肝炎患者行肝穿刺活检 ,肝组织按常规制成石蜡块 ,分别进行HE染色、苦味酸天狼红染色 ,以判断肝脏纤维化程度及炎症活动度 ;同时行PDGF -BB免疫组织化学染色 ,半定量分析PDGF-BB阳性细胞表达情况。 结果 随着肝纤维化程度和炎症活动度的加重 ,PDGF -BB在组织中的表达逐渐明显。 结论PDGF -BB是慢性乙型肝炎患者肝纤维化形成的重要的细胞因子 ,能反映肝纤维化进展状况。
Objective To investigate the role of platelet-derived growth factor BB (PDGF-BB) in the pathogenesis of liver fibrosis in chronic hepatitis B patients. Methods Forty - three patients with chronic hepatitis B underwent liver biopsy. The liver tissues were routinely made into paraffin blocks and stained with hematoxylin - eosin (HE) and picric acid respectively to determine the degree of liver fibrosis and inflammatory activity. Meanwhile, PDGF - BB immunohistochemical staining, semi-quantitative analysis of PDGF-BB positive cell expression. Results With the aggravation of liver fibrosis and inflammatory activity, the expression of PDGF-BB in the tissues gradually became obvious. Conclusion PDGF-BB is an important cytokine in the formation of hepatic fibrosis in patients with chronic hepatitis B, which can reflect the progress of hepatic fibrosis.