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[目的]分别研究鹿蹄草等4种中草药提取液对桃褐腐菌的抑制效果。[方法]采用3次浸渍提取法对中草药进行提取,通过菌丝生长速率法、单位面积产孢量计算法和凹玻片悬低法进行抑菌活性测定。[结果]在离体条件下鹿蹄草、丁香、甘草、葛根等4种中草药提取液对桃褐腐病菌营养生长、分生孢子的产生与萌发都有不同程度的抑制作用,且对菌丝细胞也有一定程度的破坏作用。鹿蹄草提取液对桃褐腐病菌的生长抑制率均达到了80%以上,丁香水蒸气提取液抑菌效率达90%以上。[结论]鹿蹄草提取液和丁香水蒸气提取液的抑菌效果较为明显,说明鹿蹄草提取液和丁香水蒸气提取液中含有抑菌成分。
[Objective] The research aimed to study the inhibitory effects of four kinds of Chinese herbal medicine extracts such as wintergreen on the brown rot fungi. [Method] The Chinese herbal medicine was extracted by 3 times immersion extraction method, and its antibacterial activity was determined by mycelial growth rate method, calculation of sporulation per unit area and concave hanging glass method. [Result] Under the ex vivo conditions, the extracts of four kinds of Chinese herbal medicines such as Hippophae, clove, licorice root and Pueraria lobata inhibited the vegetative growth and conidial generation and germination of Trichogramma halibut, and inhibited the mycelial growth Cells also have a degree of disruptive effect. Wintergreen extract against brown rot bacteria growth inhibition rate reached more than 80%, clove water vapor extract antibacterial efficiency of more than 90%. [Conclusion] The antimicrobial effect of the extract of Hippophae rhamnoides and clove water vapor was obvious, which indicated that the extracts of Hippophae rhamnoides and clove contained the antibacterial component.