作者用生物化学方法和流式细胞仪研究了两种小鼠杂交瘤细胞系167.4G 5.3和S3H5/r2bA2在适应低血清和无血清培养过程中的生理变化,比较了生长曲线、细胞代谢、抗体产生速度等指标。 试验表明:两种细胞系适应低血清或无血清培养后,对血清浓度的依赖性大大降低。适应低血清培养后的细胞再在高血清培养液中培养,其生长曲线与在低血清培养液中的生长曲线相似;适应无血清培养液OPTI The authors used biochemical methods and flow cytometry to study the physiological changes of two murine hybridoma cell lines, 167.4G 5.3 and S3H5 / r2bA2, in their adaptation to low and non-serum cultures. Growth curves, cell metabolism, antibodies Produce speed and other indicators. Experiments show that: both cell lines adapted to low serum or serum-free culture, the dependence on serum concentration greatly reduced. Adapt to low serum culture cells and then cultured in high serum medium, the growth curve and growth curve in low serum culture medium similar to adapt to serum-free culture medium OPTI