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选择7名健康老年人为对象,研究了人参皂甙Rg1对其淋巴细胞表面抗原。受体及蛋白质酪氨酸激酶(PTK)活性的作用,用间接免疫荧光法测定了CD25、CD45RA及CD45R阳性细胞百分率。单独PHA(5μg/ml)培养72小时分别为38.3%±173%、46.0%±15.1%、526%±14.4%;而用PHA(5μg/ml)和Rg_1(μg/ml)联合培养时则分别为58.0%±12.5%。64.1%±12.4%、74.1%+8.0%。两者相比分别皆有显著性(P<0.05),用ELISA法测定了经PHA及PHA+Rg_1培养30分钟和72小时细胞浆PTK的吸光度值,PHA组为0.120±0.020,PHA±Rg_1组为0.138±0.015,(P<0001)。结果证明,Rg_1显著升高PTK活性。据此得出几点结论,并讨论广Rg_1对PTK的作用机理。
Seven healthy elderly subjects were selected to study the effects of ginsenoside Rg1 on their lymphocyte surface antigens. Receptor and protein tyrosine kinase (PTK) activity, the percentage of CD25, CD45RA and CD45R positive cells were determined by indirect immunofluorescence. The results showed that PHA (5μg / ml) and Rg_1 (μg / ml) cultured for 72 hours were 38.3% ± 173%, 46.0% ± 15.1% and 526% ± 14.4% / Ml) were co-cultured, respectively 58.0% ± 12.5%. 64.1% ± 12.4%, 74.1% + 8.0%. (P <0.05). The absorbance values of cytoplasmic PTK in PHA and PHA + Rg-1 culture for 30 and 72 hours were measured by ELISA. PHA group was 0.120 ± 0.020, The PHA ± Rg_1 group was 0.138 ± 0.015 (P <0001). The results show that, Rg_1 significantly increased PTK activity. Based on this, we can draw some conclusions and discuss the mechanism of action of Rg_1 on PTK.