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以甘薯优良品种“新大紫”的茎尖培养再生植株为试材 ,经根癌农杆菌介导将甘薯块根贮藏蛋白启动子 (Sporamin promoter)调控下的 10 k D玉米醇溶蛋白基因导入到“新大紫”中 ,并获得了转化植株。甘薯品种“新大紫”再生植株的茎切段 ,与携带有表达载体质粒 p SP10 Z的根癌农杆菌菌株 LBA4 4 0 4共培养后 ,在含 75 mg/ L 卡那霉素 (Kan)的筛选培养基上可产生抗性芽 ,最高频率达 6.2 %。其中 ,32 .1%的抗性芽可在含 10 0 mg/ L Kan的培养基上生根 ,从而形成转化植株。PCR和 PCR-Southern检测证实 ,10 k D玉米醇溶蛋白基因已导入并整合到甘薯品种“新大紫”的基因组中。
In this experiment, the regenerated plantlets from the shoot tips of sweet potato variety “Xinzigazi” were used as materials to induce the 10 kD zein gene under the control of Agrobacterium tumefaciens root-in-tube storage protein promoter (Sporamin promoter) “New big purple”, and get transformed plants. The stem segments of sweet potato variety “Zizyphus jujube” were co-cultured with Agrobacterium tumefaciens strain LBA4404 harboring the expression vector plasmid pSP10Z and cultured in medium containing 75 mg / L kanamycin (Kan) The screening medium can produce resistant buds, the highest frequency of 6.2%. Among them, 32.1% of resistant shoots could be rooted on medium containing 100 mg / L Kan to form transformed plants. PCR and PCR-Southern assays confirmed that the 10 kD zein gene was introduced and integrated into the genome of the sweet potato variety “Vitex”.