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目的:利用强度不同气流吹干制备完全的深龋洞后充填窝洞,研究强气流吹干牙本质对成牙本质细胞的影响。方法:选取20颗健康成人第三磨牙,表现为无对颌或是阻生齿,均未患有牙体牙周疾患。将选取的20颗牙齿分别制备洞型致剩余牙本质厚度约0.25mm,将20颗牙齿随机分为2组,一组使用轻到中度强度气流吹干窝洞后进行充填作为对照组,另一组使用强气流。显微图像分析系统计算台盼兰染色率以判断强气流对成牙本质细胞的影响,并做统计学分析。结果:利用强气流吹干窝洞可造成龋洞对应髓室顶或髓室壁的成牙本质细胞及其突起萎缩、坏死,周围区域成牙本质细胞形态良好。结论:使用强气流吹干切割完全的牙本质可伤害成牙本质细胞,临床治疗深龋应避免使用强气流吹干生活牙本质。
OBJECTIVE: To prepare complete deep cavities by means of drying with different intensities of airflow and then filling the cavities to study the effect of strong airflow on the odontoblasts. Methods: Twenty healthy adult third molars were selected, showing no jaw or impacted teeth and no periodontal disease. The selected 20 teeth were prepared hole-induced residual dentin thickness of about 0.25mm, the 20 teeth were randomly divided into two groups, a group of light to moderate intensity air blow drying holes after filling as a control group, and the other One group uses strong airflow. Microscopic image analysis system to calculate trypan blue staining rate to determine the strong airflow on the odontoblasts, and do statistical analysis. Results: The use of strong air blow drying holes can cause cavities corresponding to medullary roof or medullary wall of odontoblasts and their processes atrophy, necrosis, the surrounding area of odontoblasts in good shape. Conclusion: The use of strong air blow to dry completely cut dentin can damage odontoblasts, clinical treatment of deep caries should avoid the use of strong airflow to dry living dentin.