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为了探索大鼠坐骨神经（躯体性）和盆神经（内脏性）内与传递痛信号有关的初级传入神经元在后根节内的分布特点，本研究采用荧光金逆行追踪与免疫荧光组化技术相结合的方法，对ＣＧＲＰ能、ＳＰ 能和ＮＯＳ样神经元在相应的后根节内（坐骨神经，Ｌ４～Ｌ６ ；盆神经，Ｌ６～Ｓ１）的分布状况进行了分析。结果表明：（１）坐骨神经和盆神经初级传入神经元中有相当数量的ＣＧＲＰ和ＳＰ样阳性细胞，与这二者相比，ＮＯＳ样细胞数量稀少；（２）盆神经初级传入神经元中ＣＧＲＰ／ＦＧ、ＳＰ／ＦＧ、ＮＯＳ／ＦＧ 双标细胞的比率高于坐骨神经，而其前两种双标细胞与各该活性物质单标细胞的比率则低于坐骨神经；（３）三种物质与ＦＧ 的双标神经元以小型为主，少有中型细胞。因为既往的研究证明，分布有大量的ＣＧＲＰ、ＳＰ、ＮＯＳ样终末的骶髓后连合核（ＳＤＣＮ）接受盆腔脏器伤害性信息传入，并且ＣＧＲＰ、ＳＰ都以外周来源为主。故本文结果进一步核实了ＳＤＣＮ 区接受来自外周的ＣＧＲＰ、ＳＰ投射，且确为经盆神经传入的细纤维。
In order to explore the distribution of primary afferent neurons involved in the transmission of pain signals in the sciatic nerve (soma) and pelvic nerve (visceral) in the dorsal root ganglion, we used fluorescence gold retrograde tracing and immunofluorescence staining The distribution of CGRP, SP and NOS-like neurons in the corresponding dorsal root ganglion (sciatic nerve, L4 ~ L6; pelvic nerve, L6 ~ S1) were analyzed. The results showed that: (1) There were a considerable number of CGRP and SP-like positive cells in the primary afferent neurons of the sciatic nerve and pelvic nerve, and the number of NOS-like cells was scarce compared with the two; (2) The ratio of CGRP / FG, SP / FG and NOS / FG double-labeled cells was higher than that of the sciatic nerve while the ratio of the first two double-labeled cells to the single labeled cells of each active substance was lower than that of the sciatic nerve. (3) Double-labeled neurons with FG are mainly small and few have medium-sized cells. Previous studies have shown that large numbers of CGRP, SP, and NOS-like terminal sacral commissural nucleus (SDCN) are involved in the transmission of nociceptive information to pelvic organs, and CGRP and SP are mainly peripheral sources. Our results further confirm that the SDCN region receives CGRP, SP projection from the periphery, and is indeed a fine fiber introduced through the pelvic nerve.