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[目的]建立兴安白头翁药材中常春藤皂苷元含量的测定方法.[方法]采用反相高效液相色谱法,色谱柱为BDS HYPERSIL C18色谱柱(4.6mm×250.0mm,5μm),流动相为乙腈∶水(85∶15),流动速度为0.5mL/min,柱温为30℃,检测波长为210nm.[结果]常春藤皂苷元的线性范围为1～100mg/L(r=0.9995),平均加样回收率为107.86%,RSD为0.78%.[结论]反相高效液相色谱法准确,专属性强,可作为兴安白头翁的含量测定方法.
[Objective] To establish a method for the determination of the content of Ivy saponin in Pulsatilla Chinensis. [Method] The chromatographic column was BDS HYPERSIL C18 column (4.6mm × 250.0mm, 5μm) with reversed-phase high performance liquid chromatography : Water (85:15), the flow rate was 0.5mL / min, the column temperature was 30 ℃ and the detection wavelength was 210nm. [Result] The linear range of Ivy saponin was 1 ~ 100mg / L The recovery rate was 107.86% and the RSD was 0.78%. [Conclusion] RP-HPLC was accurate and specific and could be used as a method for the determination of Pulsatilla.